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Test Code FS Fungal Smear, Varies

Reporting Name

Fungal Smear

Useful For

Detection of fungi in clinical specimens

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TISSR Tissue Processing No, (Bill Only) No

Testing Algorithm

For information, see Meningitis/Encephalitis Panel Algorithm.

Method Name

Calcofluor/KOH Stain

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


Specimen should arrive within 24 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

 

Specimen Type: Body fluid

Container/Tube: Sterile container

Specimen Volume: Entire collection

 

Specimen Type: Fresh tissue

Container/Tube: Sterile container

Specimen Volume: Pea size

Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.

 

Acceptable:

 

Specimen Type: Bone marrow

Container/Tube: Sterile container, SPS/Isolator system, or green top (lithium or sodium heparin)

Specimen Volume: Entire collection

 

Specimen Type: Respiratory specimen

Container/Tube: Sterile container

Specimen Volume: Entire collection

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions: Collect a random urine specimen.

 

Fresh tissue or body fluid are preferred over a swab specimen. Recovery of mycobacteria from swabs is generally very low yield. Only submit a swab specimen if tissue biopsy or fluid aspiration is not possible.

 

Specimen Type: Swab

Supplies: BD E-swab (T853)

Sources: Dermal, ear, mouth, ocular, throat, or wound

Container/Tube: Sterile, screw-capped tube containing Liquid Amies Medium with flocked swab (eg, E-Swab)

Specimen Volume: Flocked swab in 1 mL of Liquid Amies Medium

Collection Instructions:

1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.

2. Obtain secretions or fluid from source with sterile flocked swab.

3. Place flocked swab in sterile, screw-capped tube containing 1 mL of Liquid Amies Medium.

4. If smear and culture are requested or both a bacterial culture and fungal culture are requested, collect a second swab to maximize test sensitivity. Submit each swab in a separate sterile, screw-capped tube with 1 mL of Liquid Amies Medium.


Specimen Minimum Volume

Bone marrow, body fluid, or respiratory specimen: 0.2 mL; Any other specimen type: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Ambient  7 days

Reject Due To

Blood or fixed tissue
Specimen in viral transport (including but not limited to M4, M5, BD viral transport media, thioglycolate broth)
Nasal swab
Wood shaft or charcoal swab
Catheter tips
Prepared slide, glass slide, microscope slide
Stool
Boric acid tube
Aptima swab
Culture transport swab (eg, Culturette)
Reject

Reference Values

Negative

Day(s) Performed

Monday through Sunday

CPT Code Information

87206

87176-Tissue processing (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FS Fungal Smear 658-5

 

Result ID Test Result Name Result LOINC Value
FS Fungal Smear 658-5

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Report Available

1 to 2 days

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.