Sign in →

Test Code MPRP Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies

Additional Codes

Mayo Test ID COPIA Test code EPIC Test Codes
MPRP MPRP-Mycoplasma pneumoniae, Molecular Detection, PCR LAB0213009 MYCOPLASMA PNEUMONIAE, PCR

Reporting Name

M pneumoniae PCR + Macrolide Reflex

Useful For

Diagnosing infections due to Mycoplasma (Mycoplasmoides) pneumoniae

 

Assessing macrolide susceptibility

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RPMPM M. pneumoniae Macrolide Resist PCR Yes No

Testing Algorithm

If positive, Mycoplasma pneumoniae macrolide resistance will be performed at an additional charge.

Method Name

Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma (Mycoplasmoides) pneumoniae DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Swab

Supplies:

-Culturette (BBL Culture Swab) (T092)

-BD E-Swab (T853)

-Nasopharyngeal Swab (Nylon Mini-Tip Swab) (T861)

-Culture Swab-Liquid Stuarts/Single Swab (NP Swab) (T515)

-M4-RT (T605)

Sources: Throat, nasal, or nasopharyngeal

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab

Specimen Volume: Swab

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Specimen Type: Fluid

Sources: Pleural, pericardial, cerebrospinal

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

 

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Minimum Volume

Respiratory specimen: 0.5 mL
Other specimen types: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal
Port-a-Cul tube
Anaerobic fluid vials
Dry swab (no pledget or sponge)
Respiratory fluid specimens placed in VTM or placed on a swab and then into VTM (M4-RT, M4, or M5)
Body fluid specimens placed in VTM or placed on a swab and then in VTM (M4-RT, M4, or M5)
Reject

Reference Values

Negative

Day(s) Performed

Monday through Sunday

CPT Code Information

87581

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MPRP M pneumoniae PCR + Macrolide Reflex 29257-3

 

Result ID Test Result Name Result LOINC Value
SRCMP Specimen source 31208-2
62394 M. pneumoniae PCR 29257-3

Report Available

3 to 4 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.